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KMID : 0354719940180030222
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Journal of Korean Diabetes Association
1994 Volume.18 No. 3 p.222 ~ p.228
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Effect of Acute Sepsis on Erythrocyte Membrane Lipid Peroxidation in diabetic Rats
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장대영
강승식/유재명/홍성관/임성희/쵬문기/노정우/유형준/박성우
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Abstract
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Background:
@EN It has been reported that lipid peroxidationin diabetes mellitus was increased and this increase was associated with pathogenesis of diabetic complication. Also oxygen-free radicals have been shown to be elevated in infectious disease
including
sepsis. It is commonly stated that diabetic patients are more susceptible to infection. have worse prognosis, and are more resistant to treatment that nondiabetic ones. The frequent coincidences of infection in diabetes mellitus cause significant
clinical problems. However, there are few studies about lipid peroxidation in diabetes mellitus with infection. So, we investigated the effect of acute sepsis on erythrocyte membrane lipid peroxidation in streptozotocin induced diabetic rats.
@ES Methods:
@EN Sprague-Dawley rat weighing 225~336 gm were divided into 4 groups: controls (N=7); diabetic animals (N=6); septic animals (N=11); diabetic animals with sepsis (N=8). Diabetes was induced by i.p. injection of streptozotocin (55㎎/㎏body
weight)
and
it's progress was checked for 7 days. Sepsis was induced by i.p. injection of S. aureus ATCC 25923 2.0×10E14 CFU, and was confirmed by blood and peritoneal fluid culture. Erythrocyte membrane lipid peroxidation was determined by measurement of
malonyldialdehyde (MDA) reactivity (nmol/mL packed cells).
@ES Results :
@EN 1) The MDA reactivity of erythrocyte membrane in diabetic (median±median absolute deviation: 1.91±0.09nmol/mL packed cells) and septic rats (1.83±0.32nmol/mL packed cells) were higher (p<0.01) than that in normal controls
(1.15±0.26nmol/mL
packed cells). But, there was no significant difference between diabetic and septic ones.
2) The MDA reactivity of erythrocyte membrane in diabetic rats with sepsis (1.70±0.21nmol/mL packed cells) was higher (p<0.01) than that in normal controls (1.15±0.26 nmol/mL packed cells). But, there was no significant difference between
diabetic
ones with sepsis and diabetic or septic ones.
@ES Conclusion:
@EN There was no further increase in the lipid peroxidation of erythrocyte membrance from the diabetic rats with sepsis compared with diabeitc ones without it. The significance of this finding needs to be confirmed through further studies such as
quantitative lipid peroxidation measurement.
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KEYWORD
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